Understanding Punnett Squares (Opal x Castor)

This is Jack and CupCake.


And these are their kits at 2 days old. I’m taking a guess I see castor, lilac, and chocolate based kits. Some are self and some have pattern. I am honestly in hopes that one in the right picture all the way to the left is a red. Keeping fingers crossed.  😀


Here is the Punnett Square for the kits I made with Jack and CupCakes genotype based on the phenotype alone. I think this is the correct way to present a punnett square.


Ok that posted. Here goes. Ha.

This is how I understand the Letter gene series.

The A gene can only be 3 things – agouti “A”, tan “at” or self “aa”.

CupCake and Jack both have one “A” in their phenotype, the other gene is unknown – but now I have kits from them so here goes.  😯 So if one of the babies is all brown (chocolate) or a self, that would mean both CupCake and Jack need to have each contributed the “a” gene. Is this right ?

Ok, now if at least one of the kits is a self chocolate or solid color (for example) – both CupCake and Jack contributed the “a” gene meaning on the punnett square I can change both CupCake and Jacks phenotype to “Aa”. That’s how I understand it.

On to the B series. “B” black and “b” brown (chocolate) – dilute of black is blue and dilute of brown (chocolate) is lilac. Ok same thing here – if one of the kits from CupCake and Jack is brown (chocolate) then each parent had to contribute the “b” gene. This means I can change both CupCake and Jacks phenotype on the punnett square to “Bb”. That’s how I understand it.

Now the C gene, this letter series is complicated but I think I know the basics. Correct me if I’m wrong. The C series is responsible for full colors. I know CupCake and Jack are not “c” meaning REW (ruby eyed white) so no “c” in the phenotype. Also none of the kits are REW so I think it’s safe to say no “c” gene. At least so far with one breeding. Not sure what to put on the punnett square other than one “C” for both CupCake and Jack. I’m leaning toward C(chd) but I don’t know enough yet so I leave the C location alone for now.

Ok the D series is density and dilution of color. “D” dense, “dd” dilute and “Dd” dense carrying dilute. Simple enough. So if one of CupCake and Jacks kits is a lilac that means each parent contributed a “d” gene. Meaning I can change both Jack and CupCake to “Dd” on the punnett square.

And finally the E series which is responsible for extension or non-extension of color down the hair shaft. There is a whole slew of them but I figure process of elimination here. “EE” full extension of color, “ee” non extension of color, “Ee” extension carrying non extension and “enen” solid color. At this point I don’t think any of the other genes in this letter series apply – but I dunno and I’m not quite sure how “enen” fits in either with the solid color. If someone could explain that would be great.  :geek:

— about this E series – I think because I see a possible red (not sure of that either) I assume I can change both Jack and CupCakes punnett square to “Ee” ? and tag onto that “en” because the possible chocolate, lilac and red appear one color – so there would have to be a solid color gene in there somewhere. Still learning all this though.  😉

So here is the new punnett square – with what I think looking at the kits. What do you think ? Corrections ?


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(John x Jill) Jade – Solid Black – 8 weeks old.

This is a kit from the very first litter of my Breeding Project. I believe a male, but because I’m new to all this rabbit stuff – I could be wrong. I’m pretty sure Jade is a male though.

John (Black Otter) x Jill (Black Tort) = Jade aaB-C-D-Ee carries the rex gene.

This baby is friendly, used to being handled, not afraid of a dog or cat and loves to sit with us on the sofa. This baby bunny loves his people and knows the litterbox.

This bunny is listed on my For Sale page.








YAY – I also created a Weebly site for Kirst Rabbitry – Check it out !!

In addition to The Rabbit Hole discussion forum I also over the past few days created a site on Weebly for Kirst Rabbitry.

I figured having a website to showcase all my rabbits would be ideal instead of paging threw all the wordpress posts to see pictures of my rabbits.

The new website is simple and you can click the tab that interests you most like the boys or the girls or the for sale or the gallery and wallah.

I’m not sure how to link them all yet but for now the new website will do if anyone wants to see info conveniently in one place.


The Rabbit Hole Discussion Forum – Brand New – if you like talking rabbits check it out !!

The Rabbit Hole discussion forum – a place where beginners like me and experts can communicate, learn and teach one and other all about rabbits.

I decided to start The Rabbit Hole forums because I like to post pictures, video’s and just share everything about my rabbits and ask alot of questions. I noticed on some forums I’m either shunned as a fast moving beginner or am considered a spammer with all my blog posts and pictures I share. I figure the only way to remedy this is to create a forum so I and others like myself can share all the pictures and video we like of our rabbits and of course to learn and communicate without hesitation.

Please consider joining The Rabbit Hole


John and Jill’s kits at 14 days old.

Today John and Jill’s baby bunnies are 16 days old. They are growing nicely !!

They have their eyes wide open, nibbling on timothy hay and alfalfa and running circles around mom. I bought a 3 tier rabbit condo for inside the house that I figure I’d use for a housing and weaning area for the kits. I put a litter box in there to get them used to using a litter box. Of course they can only use the bottom of the condo for now so limiting space keeps them going back to the same spot to pee and poo.

I do have some interesting news about Jill and John – I now know Jill is a Mini Rex cross because breeding her to John who is a Standard Rex – she had 2 baby bunnies that have rex fur and 2 non-rex fur. So, she does carry the rex gene.

I did the Punnett Square on Jill and John to see what genotype their kits are – my conclusion on genotype is this  aaB-C-D-Ee for John and Jill’s 4 baby bunnies.

Rabbit Punnett Square

Rabbit Punnett Square

Here is what the Punnett Square dictates when breeding John to Jill. Although both loci in the B,C,D is still unknown for both Jill and John. You can see what the babies genotype is with what we do know about John and Jill.

I only have genotype for John and Jill’s visible phenotype : Please correct me if I’m wrong, still learning here. The only thing I do know  about the genotype is that John and Jill’s 4 kits will be all carrying non-extension. “Ee” Also, if  John and Jill ever give birth to Torts then I can add in “e” on John’s genotype. Same with if any dilutes show up, then I can go back and add one dilute “d” to both John and Jill’s genotype.

They are BB so Black no chocolate.
They are CC so full color, not chinchilla.
They are DD so full strength of color. So no dilute.
They are Ee so full extension of color.

Small “a” denotes self.  John’s “a” loci are known because he is an Otter so he carries two small “a”s at least one will have the attached “t”. Going by what I have written on the square for John and Jill, their babies will be dense (black based) Selfs, Otters, Torts (black non extension) and most likely Tort Otters.  Although, John and Jill didn’t have Otters, Torts or Tort Otters. There are however still many unknowns there such as whether there is dilute in the “D” loci, whether John has two non-extension or only one in the “E” loci.

For me to fill in the unknown in John  and Jill’s Punnett Square – John being an Otter, I would need to breed him to a Chocolate, something dilute, something REW or with REW to see what else is on John’s  B_C_D_ locus.

Again, I’m learning all this so if you see I have made a wrong conclusion or mistake, please leave me a comment. It would be appreciated.

I took some pictures of them on day 14. So darling !!



Maniac 2

Maniac 2

Maniac 3

Maniac 3

Maniac 4

Maniac 4